Layer 2/3 | Layer 5 | Layer 2/3 versus layer 5 | |||
Sham (n = 16) | FL (n = 16) | Sham (n = 16) | FL (n = 16) | ||
Resting potential (mV) | -67.4 ± 0.8 | -66.5 ± 0.5 | -56.6 ± 0.8 | -55.4 ± 0.8 | **** |
Input resistance (MΩ) | 434 ± 39 | 367 ± 21 | 230 ± 22 | 313 ± 36 | - |
Capacitance (pF) | 336 ± 14 | 361 ± 11 | 593 ± 40 | 530 ± 26 | **** |
Spike frequency (Hz @ 200 pA) | 19.3 ± 0.6 | 19.9 ± 0.8 | 15.4 ± 1.3 | 16.5 ± 0.8 | * |
AP #1 half width (ms) | 1.72 ± 0.6 | 1.74 ± 0.06 | 1.48 ± 0.04 | 1.51 ± 0.04 | - |
AP #3 half width (ms) | 3.21 ± 0.28 | 2.96 ± 0.17 | 2.00 ± 0.14 | 2.09 ± 0.12 | *** |
AP #1 maximum (mV) | 52.5 ± 1.0 | 53.9 ± 1.3 | 57.3 ± 1.3 | 56.4 ± 1.1 | - |
AP #3 maximum (mV) | 36.5 ± 2.7 | 38.5 ± 1.6 | 40.5 ± 3.4 | 41.6 ± 2.0 | - |
AP threshold (mV) | -36.3 ± 0.5 | -37.4 ± 0.5 | -36.2 ± 0.3 | -36.6 ± 0.6 | - |
Ih at -80 mV (pA) | 9.2 ± 2.2 | 7.5 ± 2.3 | 96.1 ± 11.6 | 84.0 ± 11.2 | **** |
-
Note: AP, action potential. Intrinsic membrane properties of pyramidal cells in layer 2/3 and 5. There were no significant differences between cells of FL and control cortex within each layer. There were significant differences between layer 2/3 and 5 pyramidal cells (right column). *P < 0.05; ***P < 0.001; ****P < 0.0001; -, not significant, P > 0.1. P values were determined for comparisons between control cells, but if no significant difference was detected between cells from FL cortex, the comparison was listed as not significant.